http://peptide-guide.com/

Melanotan One, Scenesse, Afamelanotide (M-I) Dose:

Light:  .5mg
Common:  1mg
Large:  2mg

Melanotan/Afamelanotide is a synthetic version of melanocyte stimulating hormone (a-MSH).  Afamelanotide treatment induces skin pigmentation through melanogenisis, the production of melanin.  Melanin prevents cellular damage in the skin by absorbing, reflecting and refracting light.  Melanotan reduces sun damage to UV exposed skin in those with sun allergies.

Melanotan Results: Melanotan favors production of eumelanin (black/brown) over pheomelanin (red/yellow) pigment.  Injecting this linear amino acid structure peptide offers super-physiological levels of a-MSH.  Learning the majority of secretion of a-MSH comes from the skin as opposed to the pituitary gland was a recent breakthrough in medical science.  Those with a Fitzpatrick skin type I-III classification have natural levels of a-MSH that are hardly detectable.  The fair skinned can achieve a photoprotective tan with synthetic MSH.

Research Use: The half-life of Melanotan peptides is very brief.  Afamelanotide is being developed in implant form for the ease of use in dermatology.  The implant contains 16mg which has a controlled release effecting melanin density for months.  Melanotan One is offered in research peptide form (20mg Melanotan One).  Peptide Melanotan One gives the user greater control over dosing, however requires injections and expertise.

Lifestyle: Melanocortins have the potential to be the next big thing in lifestyle drugs (Viagra, Botox, etc).  Patients who benefit from treatment have such variations when it comes to their goals, expectations and genetics.  Melanotan can be appropriately used continuously as well as intermittently.  Dose timing and missing doses are generally not of much concern.  Missing a dose and taking time off is the name of the game for the lifestyle peptide.

Recommended Therapy: Intermittent therapy, lasting 10 days (skin type III) to 6 months (skin type I) works well.  Take note how long after your Melanotan administration melanin density stays elevated.

Molecule: Two amino acids present in the bodies natural a-MSH have been changed in production of Afamelanotide creating more stablity.  Melanotan only works when injected subcutaneously.
Amino acid sequence: Ac-Ser-Tyr-Ser-Nle-Glu-His-D-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2

Approval: Melanotan is still undergoing clinical trials and has not reached approved status.  People use on the pretenses and understanding that it is for research purposes only.  Users may suffer from slight nausea and flushing after subcutaneous injection; however it is reported to be mild and pass quickly.

Safety First: Melanotan One is a selective agonist of the MC1R (Melanocortin 1 receptor) and does not have aphrodisiac effects.  Melanotan One does not cross the blood brain barrier.  It is for this reason Afamelanotide has continued clinical trials.  The fat/weight loss, appetite suppression, libido effects are found in Melanotan 2 and Bremelanotide (PT-141).

Patience: Melanotan One is less efficacious than its counterpart Melanotan 2 for achieving a dark tan.  Afamelanotide will therefore require 8-10x the dosage to see comparable results.  Melanotan One users want minimum side and peripheral effects with a focus on photoprotection.  Be patient and prepared through your Melanotan journey.

Administration of Research Melanotan
Safe and controlled administration of Afamelanotide for research is done much like that of Melanotan 2.  Melanotan One offered as a peptide in a multi-dose sterile vial is desirable.  Implants, nasal sprays and pills are not applicable/efficacious at the present time.

Melanotan Treatment
Allow time before seeing UV exposure.  Sunburn is to be avoided.

Example-  Skin type II, 180lb male
Day 1-2:  .5mg each night
Day 3-6:  1mg when convenient
Day 7-10:  2mg when convenient
Day 11 on forward:  begin dosing ~6mg/week

Day 7:  10min sun bed session.  (Cover face/genitals/sun damage, use lotion, etc)
Day 9:  10min sun bed session.
Day 11:  15min sun bed session

Dosing According to Skin Type

Fitzpatrick Skin Type I:  1mg-2mg/day.  100mg total may be necessary to achieve desired color.  UV exposure after at least 20mg in system
Fitzpatrick Skin Type II:  .75mg-1.5mg/day.  60mg should be sufficient to get color.  UV exposure after 10-20mg
Fitzpatrick Skin Type III:  .5mg-1mg/day.  A sunless tan may be realistic here
Fitzpatrick Skin Type IV and beyond:  Do not use Melanotan

Melanotan Video from Australia

Melanotan can develop a protective tan in users.  A natural tan is developed in response to cell damage caused by UV radiation.  Ultraviolet rays penetrate the upper layers of the skin, triggering a-MSH, stimulating melanin production.  Melanin deposits act as a natural sunblock at the cellular level.  Melanotan tanning injections may offer a way to bring upon a safe, cosmetic tan with less damage.

Melanotan Results

Reconstituting

Swab the stopper of both your M-I vial and the vial of the dilutent (bacteriostatic water) with an alcohol swab.

Take a syringe and draw up your bact water. The amount isn’t critical, other than making sure you know exactly how much you have used. The best rule of thumb is choose an amount that will make measuring the final product easy.

Example- 2ml(cc) per 20mg vial of M-I would mean each 10 marks on a U100 insulin syringe would equal 1mg of Melanotan (M-I)
Example- 1ml(cc) per 10mg vial of M-I would mean each 10 marks on a U100 insulin syringe would equal 1mg of Melanotan (M-I)

Avoid shooting the dilutent directly on the lyophilized powder.  The powder should almost instantly dissolve.  Gently swirl until the liquid is clear if powder did not dissolve upon contact.

Store your unused M-I in the refrigerator.  Once reconstituted, the Melanotan should be good for 4-6 weeks in the refrigerator.

Measuring

After successfully reconstituting your peptide, now measure the desired amount out for injection. You will want to use a U100 insulin syringe to draw out your M-I.

cont’d

TV3 reports on Melanotan II and how the tanning peptide injection effects

melanin as well as aphrodisiac/libido enhancement & appetite suppression:

Melanotan II Instructions
 
A compilation of information regarding how to use MT-II for research. MT-II is an analog of the peptide hormone alpha-melanocyte stimulating hormone (α-MSH). This hormone acts on melanocytes to stimulate melanin production. Melanin is your pigment responsible for your tan. MT-2 out competes a-MSH for binding to melanocyte receptors in humans and other mammals because it has a binding affinity over 1000x. This is the reason why the fair skinned CAN reach a natural tan.
 
Please pursue information regarding the usage of these products from your own research, academic journals, or the research of your in-house scientific team. Products are sold for research purposes are not for human consumption. Remember when you contact these types of stores/sponsors not to relate the questions or topics to human consumption or they tend not to answer. Always inquire in a research type question. Cause after all, we know that this is for your experiments and not for human consumption.
 
Mixing: Add bacteriostatic water to the vial when you are ready to begin journey. The peptide starts to degrade after being reconstituted.
Comparable homemade video:

 
I recommend adding 100 units of water to the vial. This will minimize the volume that you have to inject and will simplify the arithmetic.
 
http://peptidecalculator.com/
 
1ml syringe, 1ml bact water to reconstitute
Calculations (given you want a .5mg dose):
step 1= 1ml
step 2= 10mg MT-II
step 3= 1ml bact water
step 4= 500mcg dose
2-3 ticks on your insulin pin (1/20th a syringe full)
 

Some people prefer to add more dilutent which works fine, take note of the volume increase.
 
Needles: 29-31 gauge X 1/2″, 1 CC (100 unit). That is a typical insulin needle. Once you get your technique perfected, it is almost painless. [url=http://peptidecalculator.com/subq.html]More info[/url]
 
Starting dose: Your first injection should be a very small dose, for example .25mg. See how you react. Goal should be to feel nothing. Dose after dinner, before bed. Any dosing chart stating that you should take a high dose (according to your weight) is outdated and potentially dangerous.
 
Loading dose: I recommend loading with 0.5-1mg once a day. People who have used doses in this range generally report getting excellent results. Don’t worry if you miss occasional days. It will not make much difference, focus on the cumulative effects. [url=http://melanotanhq.com/forum/index.php?topic=34.0]Nausea and yawning were frequently reported side effects due to Melanotan II[/url]
 
Maintenance dose: Maintenance is taking doses less frequently than daily to avoid becoming darker than you want. Yes, that will happen. With enough UV, you will get much darker than you have even been before. I recommend .5mg every mon. and thurs. In my case, I tan on Weds. in between the two doses, then in the weekend during the summer I am in the sun. It works great.
 
UV Radiation: MT-II is not a good sunless tanner. You need UV (from sun or a tanning bed) to develop a tan. Without UV almost nothing happens. In other words, NO UV = NO TAN. Well, user will pigment…. But if you have loaded for a full month and then start UV exposure, you (and your friends) will be astounded by how fast you tan and how dark you get.
 
Watch yourself: Your tan can sneak up on you. A tan generally sets in 3 days after UV rays

Avoid burning: You are protected from burning mostly by your tan, not the melanotan II that you have taken. Therefore, don’t overdo the rays at first. Start with only as much UV that you could tolerate without burring before you started melanotan II. Then gradually increase your exposure. It shouldn’t take many weeks before you can tolerate many hours of strong sun without burning…which truly is incredible for those who have never experienced!
 
Continue your regular daily dose until you have reached your desired tan and do not want to become darker. Then cut injection frequency to once every 2, 3, 4, or even 7 days. You will have to experiment to find the frequency that gives you the tan that you want.
 
After mixing with water, it should be refrigerated. Not a problem to store 8 weeks
 
Melanotan:

 
Storage: It is a very durable peptide. Before it is mixed with water, melanotan II is quite stable and can last for months at room temperature.
 
Do you have to inject MT-II?
Yes. The best, most efficient method of administering melanotan is via sub-q injection. Nasal sprays do not work.


 
Note: There are many things you will need to consider before experimenting with this peptide. MT-II is not an approved drug (anywhere to my knowledge). MT-II is legal to buy, possess, etc. Variables such as skin type and individual goals need assessment, create a user log. When you start using melanotan to tan keep in mind that tanning is literally a side effect, not the objective. Melanotan is not going to color your skin, it is going to make your own skin create its own tan and that in turn creates UV protection. If you are looking to be some bronzed beach god with perfectly uniform and specific color then you are better off to going to mystic tan.
 
Know your skin type: Knowing your skin type will help you create a user log. There are 10s of thousands of melanotan users worldwide who share the experience

Melanotan is best suited for the folks with skin types I & II
 
How should I dose MT-II?
Start out with small doses and build up. A typical starting dose is around .25mg and max dose reaching 1mg. You can look at it as being as simple as that.
 
Things to consider:
There is no magic pill or formula. Few dermatologists know about melanotan. The skin is a large, unpredictable organ. Feel comfortable and confident with MT-II before use. Check out as many before and after photos and user logs as you can.

 
How much should I buy and how long will it last?
 
Skin type I: 30-50mg
Skin type II: 20-30mg
Skin type III: 10mg
Should last entire summer or season
 
Melanotan and finances?
 
Is melanotan cost prohibitive? Absolutely. There is no reason any melanotan user should be on a budget. At the same time, MT-II is a valuable investment for the prime candidate.
Initial investment can be $150-200 for product, $90 for tanning salon (you know the fees, commitment,lotions, etc add up). HOWEVER, melanotan has a lasting effect.
As a user for the past 4 years I will paint a rough picture of my expenditures as I remember them:
2005: 30mg first purchase, tanned in the sun…learned a bit about my skin type I body
arrived at a tan 3-4 weeks later and was blown away. Must have bought another 20mg and spent $300 total
2006-2007: Probably invested in 100mg over the course of those two years…vacationed a lot and spend ~$600 on melanotan and brief months at the salon
2008: 30-40mg used, very small doses required for results this year ($200?)
2009: 20-30mg …so maybe $100
Future I anticipate using 1-2 vials/yr.
 
How soon will I begin to see results?
 
You should notice a change in your skin tone after three to four weeks. If you have freckles, expect them to get darker before your actual skin color changes.
 
How long will tan last?
A tan developed using melanotan II lasts much longer than an ordinary tan. A well-tanned person returning from a beach holiday will lose most of the tan in a month if they stop getting sun. But if they had been using melanotan II and continued on maintenance after returning, they would still have most of their tan 3 months later.
 
Side effects of MT2?
 
Possible short-term side effects you should know about, including: nausea, appetite loss, facial flushing and increased libido. These may be noticeable during the first few days of treatment but should taper off.
 

http://melanotanhq.com/forum/index.php

Clinuvel’s CUV1647 (formerly Epitan)

Video Melanotan One -- Afamelanotide: